Learn more about SpheroSeev

FAQ's

SpheroSeev is easy to use and modular. You simply mix SpheroSeev with cells and culture on ultra-low attachment plates to generate the desired amount and size of your spheroids. With SpheroSeev you can generate more relevant data from your 3D models, which will save you time and effort.

1. Physiological relevance – Coated hydrogel plates generate a topography that enables spheroid formation, but cells within the spheroid format don’t have optimal accessibility to nutrients, oxygen, drugs and waste release because the spheroid grows in a very dense format. Due to SpheroSeev’s porous structure, cells within the spheroid are spread apart and have a more physiological structure.
2. Easy to use – As opposed to hydrogels that need slow thawing and low temperature conditions to avoid fast gelation during gel preparation and seeding, SpheroSeev is used at room temperature without handling concerns.
3. Animal free – SpheroSeev is not generated from a tumor extract, but is generated in a controlled bioreactor system. It is animal-free and has very high batch-to-batch consistency.

At Seevix, we have cultured the following cell types using SpheroSeev:
Primary rat hepatocytes
HepG2 – human liver cancer cell line
Panc1 – human pancreatic cancer
C3A – human hepatocellular carcinoma cells
NIH3T3 – mouse fibroblasts
L929- mouse connective tissue
RIN-m – rat pancreas/islet cell tumor
CT26 – murine colorectal carcinoma
DU145 – human prostate cancer (derived from brain metastasis)
hMSC – human adipose derived mesenchymal stem cells

Additional cells used by our customers:
GBM NCH441 cell line – glioblastoma multiforme
HT1080 – human fibrosarcoma
HCT116 – human colorectal carcinoma
EOC – epithelial ovarian cancer
SW480 – colon adenocarcinoma

Yes. This is an additional SpheroSeev benefit. You can calibrate the proportion of SpheroSeev: cell quantity according to cell type, initial number of cultured cells and desired spheroid size.

If you are using ULA plates, it is possible to centrifuge the whole plate. Actually, it will expedite the formation of aggregate.

SpheroSeev is stable at room temperature, and even stable between -200 and +230 degrees Celsius.

When too much SpheroSeev is added to the seeded cells, a number of small spheroids may be formed instead of a single spheroid. If a single spheroid is desired, it is suggested to first calibrate the concentration of SpheroSeev required for the formation of a single spheroid.

If spheroids are close enough to each other and cells are proliferating, fusion of the spheroids may occur.

Spheroid formation time depends on the type of cells. SpheroSeev may slightly prolong spheroid formation time.

We recommend replacing 50% of medium during each maintenance treatment. Maintenance frequency depends on cell type. For proliferating cells, media should be supplemented with 2µg/ml.

Yes, by trypsinization. Please visit our knowledge center for a detailed protocol.

Yes, please contact technical service, and we will adapt a protocol to your work process.

SpheroSeev fibers are biodegradable and non-toxic, and no adverse effect has been observed based on our initial in vivo studies in mice.

Yes. Please contact us and we will be happy to assist you, making use of our experience in this field.

We use a variety of fluorescence staining methods such as calcein AM for staining viable cells and propidium iodide for staining dead cells.

Fibers inside the spheroid can be fluorescently labeled with thioflavin S, a fluorescent dye that changes its excitation/emission spectra when bound to beta sheet rich structures, which are abundant in SpheroSeev.

Yes. Quantitative techniques based on luminescence or fluorescence quantitation of cellular ATP or cell metabolic activity (e.g., resazurin sodium salt) can be used to measure viability.

SpheroSeev fibers are recognized by an anti-His tag antibody.

It usually arrives within a few days.

Via a carrier with tracking.

You receive a vial of our biopolymer (SpheroSeev) dispersed in aqueous solution.

To prevent contamination, we recommend that you store the vial at 40C degrees after opening.

Before being opened, the vial has a shelf life of up to 3 years. Once opened, we recommend that you use the contents of the vial within a year.