SpheroSeev
Improve your spheroid, organoid or co-culture model
SpheroSeev
Improve your spheroid, organoid or co-culture model
using our spider silk biomimicry technology
SpheroSeev is a recombinant bio-polymer fiber an analog of the spider silk protein, providing mechanical support and ECM-like environment for organoid formation in a consistent and reproducible manner.
Better Viability, Longevity and Functionality
Confocal image of a spheroid containing SpheroSeev fibers (green) with cells (blue).
Confocal image of a stained SpheroSeev fibers.
Customers Reviews
I have used “Spheroseev” fibers to make HT1080 cancer cells spheroids, and it worked really well. I could find homogenous size distribution, and most of the spheroids are viable. I recommend using this product.
—
Alakesh Das, PhD
Irit Sagi Lab
Dept of Biological Regulation
Weizmann Institude of Science
We used Spheroseev product in order to grow epithelial ovarian cancer (EOC) spheroids from human ovarian cancer cell line – Ovcar3. In the presence of the Spheroseev, the viability of the spheroid increased significantly. In addition, the product improved the sensitivity of the cytotoxicity assay (using chemotherapy and Ovcar3 spheroids). Therefore, I would like to recommend the use of this product in a routine growth of EOC spheroids.
—
Dr Dalit Landesman-Milo PhD
Lab manager, Prof Peer laboratory for Precision Nanomedicine,
Tel-Aviv University
I used “SpheroSeev” for primary glioblastoma cell culture. It helps to form nice and stable tumorspheres, which I never achieved before. With other kinds of matrix, my GBM cell always tends to differentiate, but “SpheroSeev” keeps the stemness of the cells. Great product!
—
Xue Sun
Ph.D. Student, Oren Ram Lab
Hebrew University
Human normal primary spheroids production using “SpheroSeev”, dramatically increased viability.
—
Orit Harari-Steinberg, Ph.D, Senior
Researcher & Lab Manager
Regenerative Medicine Research Lab
Pediatric Stem Cell Research Institute
Sheba Medical Center
“SpheroSeev” 3D matrix really works well. I could find a nice spheroid. Product was used with colorectal cancer cell lines. I had used different matrixes previously from different companies but I should say from my experience that “SpheroSeev” works better than regular spheroid matrix. I like it!
—
B.N.Nishanth, Ph.D
Lab of Prof. Yosef Yarden
Dept. of Biological Regulation
Weizmann Institute of Science
Improve your chances of successful research:
SpheroSeev combines the advantages of scaffold-based and scaffold-free technologies.
SpheroSeev fibers’ nano and micro dimensions are similar to the natural ECM, providing a tissue-like environment for cells.
SpheroSeev fibers have high porosity, enabling better access to oxygen and nutrients, thereby supporting prolonged viability and functionality.
SpheroSeev fibers have extra strength and elasticity, providing mechanical cushioning and protection to individual cells within the organoid.
Applications
Drug discovery Stem cells research Cancer research Tissue engineering
3D bioprinting
Applications
Drug discovery
Stem cells research
Cancer research
Tissue engineering
3D bioprinting
Results in just a few days!
SpheroSeev fibers’ nano and micro dimensions are similar to the natural ECM, providing a tissue-like environment for cells
Spheroid created with primary hepatocytes (left) compared with larger spheroid created with hepatocytes and SpheroSeev (right).
- Confocal image of a stained SpheroSeev fibers
- TEM image of microfiber comprised of 10 nm nanofibers (insert)
Adipose-derived mesenchymal stem cells (MSCs) cultured as spheroid with SpheroSeev demonstrate tissue-like spread morphology and higher viability than spheroid without SpheroSeev. [Green – viable cells, Red – dead cells]
L929 cell line cultured as spheroid with SpheroSeev demonstrates delayed necrotic core formation and higher viability.
[Stained with PI and CalceinAM] [Green – viable cells, Red – dead cells]
ATP quantification test on primary rat hepatocytes shows that after 40 days spheroids with SpheroSeev have the same viability levels as the control spheroids in their first two weeks of culture.
SpheroSeev increases rat hepatocyte spheroid longevity for over 41 days compared with non-SpheroSeev spheroids, which disintegrate after ~20 days.
Albumin secretion test on primary rat hepatocytes shows that even after 20 days the spheroid with SpheroSeev retains good functionality while the control tissue shows no activity at all.
ATP quantification test on DU145 spheroids show that ATP level is higher when SpheroSeev concentration is greater.
hMSCs spheroids with and without SpheroSeev were cultured in adipogenic/osteogenic differentiation medium for 19 days
[Stained with oil red and alizarin red respectively]
SpheroSeev rat hepatocyte spheroids have higher basal and induced activity compared with control spheroids.
Use SpheroSeev to better mimic natural morphology and behavior of cells, increase the productivity of your research, and reduce overall study costs and time
Here’s more of why to use SpheroSeev in your research:
- Easy to use (a 5-minute process)
- Long shelf life and sterile (up to 1 year)
- Maximum flexibility to manipulate the 3D cell culture environment for different needs
- High consistency for reproducible cell-based assays
- Minimal protocol adaption to switch from 2D or currently used 3D
- Compatible with automated handling and imaging equipment
- Does not degrade or undergo alteration over the course of an experiment
Try out SpheroSeev with almost no effort!
Get a sample (shipping fee only)
Add the protein fiber to an existing experiment (a 5-minute protocol)
Discover the benefits of SpheroSeev: within days your organoid will be bigger and more vital and will survive longer
Try It Today
In order to make the decision of trying SpheroSeev in your own lab experiments and find it effective to your research, we have created a sample that you can try with almost no effort or meaningful costs.
The sample of [specs] costs only 300$(to be decided – less than 300$).
If you’d like to order the sample or in any other case, please fill up this form and our representative will contact you soon.
Try It Now
AND GET THE RESULTS YOU NEED!
Fill out this form to get a SpheroSeev sample or ask questions. Our representative will contact you as soon as possible
About Seevix
Spidersilk Innovations
Based on over a decade of academic research performed at the Hebrew University, Seevix was founded in 2014 to revolutionize material science by using spider silk’s unique natural properties to create high-performance products and materials.
We are passionate about science. We employ a remarkable multidisciplinary R&D team of biologists, chemists and engineers to ensure we remain a cut above the rest.
Our Address: Minrav Building, Biotechnology Park Hadassah Ein Kerem Campus Jerusalem, Israel 9112001
